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MDCAT
Biology
2012

Biology · Biotechnology

Work through this past-paper style MCQ, then read the full explanation. Practice more biology questions on mMCQ with adaptive practice and topic analytics.

Question

Restriction enzymes are of great use in genetic engineering because:

Options
  • A

    They cut DNA at specific base sequences

  • B

    They cut DNA at multiple random sites

  • C

    They help in joining the pieces of DNA

  • D

    They are a type of nuclease

Explanation

Restriction enzymes, also known as restriction endonucleases, are proteins that cut DNA at specific sequences called restriction sites. This ability to make precise cuts at known locations makes them invaluable tools in genetic engineering, allowing for the targeted modification of genetic material. This specificity differentiates them from other enzymes that may cut DNA randomly or serve different functions, such as DNA ligases which are used to join DNA fragments. Options B, C, and D do not accurately describe the precise and sequence-specific cutting function of restriction enzymes, which is critical for their application in genetic engineering.

Restriction enzymes are specialized proteins that cut DNA at specific sequences, known as restriction sites. This precise cutting ability is crucial for genetic engineering applications, allowing scientists to insert, remove, or replace segments of DNA with precision.

This is incorrect because restriction enzymes specifically recognize and cut at particular sequences, not randomly. Random cutting would not be useful for precise genetic engineering.

Restriction enzymes do not join DNA; they only cut. DNA ligases are the enzymes responsible for joining DNA fragments together.

While restriction enzymes are a type of nuclease (enzymes that cut nucleic acids), this answer does not address their specific role in genetic engineering, which involves precise cutting at defined sequences.

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Tagged under Biology · Biotechnology · 2012