Biology · Biotechnology
Work through this past-paper style MCQ, then read the full explanation. Practice more biology questions on mMCQ with adaptive practice and topic analytics.
Restriction enzyme EcoR1 cuts DNA to produce:
- A
Blunt ends
- B
Non-palindromic ends
- C
Sticky ends
- D
Split ends
EcoRI is a restriction enzyme. Restriction enzymes either make staggered cuts or blunt cuts. A staggered cut is one in which the duplex fragment shows single-stranded projected ends called sticky ends. Blunt cuts do not produce sticky ends.
EcoRI is a type that produces sticky ends thus this option is incorrect.
Restriction enzymes produce palindromic sequences thus this option is incorrect.
The restriction enzyme EcoRI cuts DNA to produce sticky ends with 5' overhangs of AATT. It recognizes the sequence GAATTC and makes staggered cuts between the G and A bases on both strands of the DNA double helix
Split ends are not related to the question.
Tagged under Biology · Biotechnology · 2017