Biology · Biotechnology
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Bacterial cells take up recombinant plasmids when they are treated with:
- A
CaCl2
- B
NaCl
- C
KCl
- D
NaOH
CaCl2: This option is the correct answer. Bacterial cells are often treated with a solution of calcium chloride (CaCl2) during a process called "calcium chloride transformation" to increase their ability to take up recombinant plasmids. The treatment with CaCl2 helps to create temporary pores in the bacterial cell membrane, allowing the plasmids to enter the cells.
This option is the correct answer. Bacterial cells are often treated with a solution of calcium chloride (CaCl2) during a process called "calcium chloride transformation" to increase their ability to take up recombinant plasmids. The treatment with CaCl2 helps to create temporary pores in the bacterial cell membrane, allowing the plasmids to enter the cells.
This option is not the correct answer. Sodium chloride (NaCl) is not typically used to treat bacterial cells for plasmid uptake. NaCl is a common salt used in various laboratory procedures and for maintaining osmotic balance in biological systems but is not directly involved in plasmid uptake.
This option is not the correct answer. Potassium chloride (KCl) is not typically used for treating bacterial cells to facilitate plasmid uptake. KCl is also a salt commonly used in laboratory procedures, but it does not have the same effect as CaCl2 in creating temporary pores in the cell membrane.
This option is not the correct answer. Sodium hydroxide (NaOH) is a strong base that is sometimes used to lyse cells and release cellular contents, but it is not used for plasmid uptake in bacterial cells. NaOH treatment would likely cause damage to the cells rather than facilitate plasmid uptake
Tagged under Biology · Biotechnology · 2015