Protein synthesis and secretion pathway Steps:

• Transcription of the enzyme's gene occurs in the nucleus to produce mRNA.
• mRNA travels to the rough endoplasmic reticulum, where ribosomes translate it into the protein.
• The newly synthesized protein moves to the Golgi body for processing, sorting, and packaging into vesicles.
• Vesicles fuse with the cell surface membrane, releasing the enzyme outside the cell.

Why C is correct:

• C (3→4→2→1) matches the eukaryotic secretory pathway, where nucleus initiates via transcription, followed by translation in rough ER, modification in Golgi, and exocytosis at the plasma membrane.

Why the others are wrong:

• A begins with cell surface membrane (1), the final secretion step, ignoring synthesis.
• B starts with Golgi body (2), bypassing nucleus and rough ER for initial synthesis.
• D initiates with rough endoplasmic reticulum (4) before nucleus (3), but transcription must precede translation.

Final answer: C