DNA template stop triplet determines tRNA anticodon sequence Steps:

• Identify the DNA stop triplet ATC as the template strand sequence read 3' to 5' (3'-ATC-5').
• Transcribe to mRNA stop codon: complementary bases yield 5'-UAG-3'.
• Construct tRNA to recognize UAG codon and insert new amino acid.
• Anticodon pairs antiparallel to codon: 3'-AUC-5' (RNA version of DNA template ATC, T→U).

Why B is correct:

• AUC is the 3'-5' anticodon sequence that base-pairs with UAG codon, per Watson-Crick rules.

Why the others are wrong:

• A: ATC is DNA template sequence, not RNA anticodon.
• C: TAG is coding strand DNA for UAG, not anticodon.
• D: UAG is mRNA stop codon, not anticodon.

Final answer: B