Hydrolysis and Neutralization for Non-Reducing Sugar Detection

Steps:

• Conduct initial Benedict's test; negative result (blue) indicates non-reducing sugars.
• Boil sample with dilute HCl to hydrolyze polysaccharides into monosaccharides.
• Observe blue color confirms no immediate reduction.
• Neutralize with sodium hydrogencarbonate before second Benedict's test.

Why C is correct:

• Hydrolysis with HCl breaks glycosidic bonds in non-reducing sugars (e.g., sucrose → glucose + fructose), enabling detection; neutralization removes excess acid to prevent false negatives in Benedict's reagent.

Why the others are wrong:

• A: Lacks boiling for full hydrolysis and neutralization; "negative result" is vague and incomplete.
• B: Neutralization before acid hydrolysis is incorrect order, as acid must act first.
• D: Omits neutralization after acid treatment and uses "warm gently" instead of boiling, insufficient for hydrolysis.

Final answer: C