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A Levels Biology (9700)•9700/13/O/N/22
Question 6 from 9700/13/O/N/22

Explanation

Biochemical tests identify reducing sugars and lipids

Steps:

  • Benedict's test produces brick-red precipitate with reducing sugars due to reduction of Cu²⁺ to Cu₂O.
  • Ethanol emulsion test turns cloudy when lipids dissolve in ethanol and form an emulsion in water.
  • Brick-red result confirms reducing sugars; cloudiness confirms lipids.
  • No other tests mentioned, so sample contains only these molecules.

Why A is correct:

  • Benedict's detects reducing sugars (e.g., glucose) via color change; ethanol test detects lipids via emulsion, matching both observations.

Why the others are wrong:

  • B: No test (e.g., biuret) indicates proteins.
  • C: Starch gives no Benedict's reaction; no protein test.
  • D: Non-reducing sugars (e.g., sucrose) yield no Benedict's color change.

Final answer: A

Topic: Testing for biological molecules

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