A Levels Biology (9700)•9700/11/O/N/22

Explanation
Interpreting biochemical test results for sugars and starch
Steps:
- Iodine test shows blue-black color, confirming starch presence.
- Benedict's test on original solution is negative (no red precipitate), ruling out reducing sugars.
- After acid hydrolysis, Benedict's test turns positive, indicating non-reducing sugars.
- Biuret test is negative (no violet color), confirming no proteins.
Why A is correct:
- Non-reducing sugars give negative Benedict's initially but positive after hydrolysis (breaking into reducing monosaccharides), and starch tests positive with iodine.
Why the others are wrong:
- B: Includes protein, but Biuret test is negative.
- C: Includes reducing sugar, but initial Benedict's test is negative.
- D: Omits non-reducing sugar, but hydrolysis confirms its presence.
Final answer: A
Topic: Testing for biological molecules
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