A Levels Biology (9700)•9700/13/O/N/21

Explanation
Biuret test detects proteins from amino acid monomers with carboxyl groups
Steps:
- Recall biological monomers with carboxyl groups: amino acids (-COOH in proteins) and fatty acids (in lipids).
- Match tests to biomolecules: biuret detects proteins via peptide bonds; Benedict's detects reducing sugars (no carboxyl in standard monomers); ethanol emulsion detects lipids.
- Evaluate choices: only biuret directly identifies proteins with relevant monomers; combinations including Benedict's add irrelevant sugar test.
- Select single test option that fits without extraneous ones.
Why the correct option is correct:
- Biuret test identifies proteins, polymers of amino acids that each contain a carboxyl group (-COOH), confirming the molecule's monomeric composition.
Why the others are wrong:
- A: Benedict's tests reducing sugars like glucose, whose monomers lack carboxyl groups.
- B: Includes Benedict's (for sugars without carboxyl monomers) alongside ethanol (for lipids).
- D: Includes Benedict's (irrelevant for carboxyl-containing monomers) alongside biuret.
Final answer: C
Topic: Testing for biological molecules
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