Hybridoma Technology for Monoclonal Antibodies

Steps:

• Recall that monoclonal antibodies are produced via hybridoma technology, involving fusion of immune and cancer cells.
• Identify the immune cells: activated B-lymphocytes differentiate into plasma cells that secrete specific antibodies.
• Identify the cancer cells: myeloma cells provide immortality for continuous antibody production.
• Fuse plasma cells with myeloma cells to create hybridomas that proliferate and secrete monoclonal antibodies.

Why C is correct:

• In hybridoma technique, plasma cells (antibody-secreting B-cell derivatives) fuse with myeloma cells (immortal cancer cells) to form hybridomas, as defined in immunology protocols.

Why the others are wrong:

• A: Antigens stimulate immune response but are not fused; hybridomas are the result, not the input.
• B: T-lymphocytes handle cell-mediated immunity, not antibody production; B-lymphocytes are used instead.
• D: Hybridomas produce antibodies but are not fused with them; fusion creates the hybridomas.

Final answer: C