A Levels Biology (9700)•9700/13/O/N/20

Explanation
Identifying visible cell structures in plant cell electron micrograph
Steps:
- Locate the large, double-membrane organelle with nucleolus and chromatin: identifies nucleus (1).
- Spot small, dense granules (10-30 nm) free in cytoplasm or on rough ER: confirms ribosomes (4).
- Identify narrow channels (20-50 nm) through cell walls connecting adjacent cells: reveals plasmodesmata (6).
- Absence of green plastids with thylakoids rules out chloroplasts; no tubular 25-nm filaments excludes microtubules; lack of double-membrane cristae eliminates mitochondria.
Why B is correct:
- Plasmodesmata are plant-specific cytoplasmic connections visible in EM as desmotubules linking cells, alongside universal nucleus and ribosomes.
Why the others are wrong:
- A: Chloroplasts (2) absent; no stacked thylakoid membranes shown.
- C: Chloroplasts (2) and microtubules (3) missing; no 25-nm hollow tubes or green organelles.
- D: Chloroplasts (2) not present; micrograph lacks photosynthetic structures.
Final answer: B
Topic: The microscope in cell studies
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