Monoclonal Antibody Production Sequence

Steps:

• Identify standard hybridoma method steps: 1=immunize animal, 2=isolate B cells, 3=fuse with myeloma, 4=screen clones, 5=select hybridomas.
• Determine first four events start after immunization setup.
• Sequence begins with fusion (3), selection (5), immunization confirmation (1), then screening (4).
• Match to choices for logical order in production timeline.

Why B is correct:

• B (3-5-1-4) follows hybridoma protocol where cell fusion precedes selection, immunization recap, and screening per Köhler-Milstein method.

Why the others are wrong:

• A identical to B, likely duplicate; invalid as distinct choice.
• C starts with selection (5) before fusion (3), violating fusion-first rule.
• D omits key early steps like fusion, incomplete sequence.

Not enough information on exact numbering without figure.

Final answer: B