Protein Secretion Pathway in Eukaryotic Cells Steps:

• Amino acids enter the cell across the cell surface membrane via transport proteins.
• Ribosomes on the rough endoplasmic reticulum assemble amino acids into the polypeptide chain of the digestive enzyme.
• Transport vesicles move the protein from rough ER to the Golgi body for modification and sorting.
• The Golgi body packages the enzyme into secretory vesicles, which fuse with the cell surface membrane for export.

Why B is correct:

• Matches the endomembrane system pathway where proteins for secretion are synthesized in rough ER, processed in Golgi body, stored/transported in secretory vesicles, and released via cell surface membrane exocytosis.

Why the others are wrong:

• A: Places Golgi before rough ER, but protein synthesis must precede Golgi modification.
• C: Positions secretory vesicle before Golgi and rough ER last, reversing the synthesis-to-processing order.
• D: Begins with Golgi, ignoring that amino acid incorporation into protein starts at rough ER.

Final answer: B