Competitive Inhibition Alters Apparent K_m in Lineweaver-Burk Plot

Steps:

• Plot 1/[S] vs. 1/v data for inhibited reaction to get Lineweaver-Burk line.
• Identify x-intercept as -1/K_m^{app}, where slope increases due to inhibitor.
• Estimate x-intercept from plot at approximately -4.35, so K_m^{app} = 1/4.35 ≈ 0.23 mol dm^{-3}.
• Confirm using y-intercept for V_max, which remains unchanged in competitive inhibition.

Why B is correct:

• Apparent K_m = K_m (1 + [I]/K_i); value 0.23 matches calculated increase from uninhibited K_m ≈ 0.11.

Why the others are wrong:

• A is uninhibited K_m, not accounting for inhibitor effect.
• C overestimates by misreading plot intercept.
• D confuses with possible V_max or unrelated value.

Final answer: B