Monoclonal Antibody Production via Hybridoma Technology Steps:

• Immunize a mouse with HCG antigen to stimulate B-cell antibody production (statement 1).
• Isolate spleen B cells and fuse with myeloma cells to form hybridomas.
• Screen hybridomas for specific anti-HCG antibody secretion and clone positive ones (statement 3).
• Select and culture the hybridoma line producing the desired monoclonal antibody.

Why C is correct:

• Monoclonal antibodies are produced by hybridoma technology, where immunization (1) and hybridoma screening/cloning (3) are essential steps, as defined by Köhler and Milstein's 1975 method.

Why the others are wrong:

• A includes 2, which likely misstates cell fusion or selection, omitting key hybridoma details.
• B excludes 3, ignoring the critical screening and cloning for specificity.
• D excludes 1, bypassing initial immunization needed to generate antigen-specific B cells.

Final answer: C