A Levels Biology (9700)•9700/13/M/J/21

Explanation
Calibrating eyepiece graticule using stage micrometer at ×100 magnification Steps:
- Identify stage division size: 0.1 mm, corresponding to 100 eyepiece units in the diagram.
- Convert to µm: 0.1 × 1000 = 100 µm (real stage distance).
- Divide by eyepiece units: 100 / 100 = 1 µm per unit (image plane size).
- Adjust for magnification: 1 / 100 = 0.01 µm (actual real size per unit).
Why C is correct:
- It follows the calibration formula: (stage size in mm × 1000) / eyepiece units / magnification, yielding real size in µm from the magnified image.
Why the others are wrong:
- A: Uses extraneous "100 by 0.1" step and misapplies multiplications, inflating the value.
- B: Converts and divides by units but skips magnification adjustment, overestimating real size.
- D: Applies incorrect divisions without proper conversion or magnification factor, yielding tiny erroneous value.
Final answer: C
Topic: The microscope in cell studies
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