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A Levels Biology (9700)•9700/13/M/J/21
Question 2 from 9700/13/M/J/21

Explanation

Calibrating eyepiece graticule using stage micrometer at ×100 magnification Steps:

  • Identify stage division size: 0.1 mm, corresponding to 100 eyepiece units in the diagram.
  • Convert to µm: 0.1 × 1000 = 100 µm (real stage distance).
  • Divide by eyepiece units: 100 / 100 = 1 µm per unit (image plane size).
  • Adjust for magnification: 1 / 100 = 0.01 µm (actual real size per unit).

Why C is correct:

  • It follows the calibration formula: (stage size in mm × 1000) / eyepiece units / magnification, yielding real size in µm from the magnified image.

Why the others are wrong:

  • A: Uses extraneous "100 by 0.1" step and misapplies multiplications, inflating the value.
  • B: Converts and divides by units but skips magnification adjustment, overestimating real size.
  • D: Applies incorrect divisions without proper conversion or magnification factor, yielding tiny erroneous value.

Final answer: C

Topic: The microscope in cell studies

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