A Levels Biology (9700)•9700/13/M/J/21
Explanation
Non-immobilized enzymes at high temperature maximize yield for thermophilic types Steps:
- Identify enzyme type: Assume thermophilic, with optimal activity at 75°C.
- Consider immobilization effect: It enhances thermal stability but causes diffusion limitations, reducing substrate access.
- Evaluate temperature impact: 75°C boosts reaction rate via Arrhenius equation, increasing yield until denaturation.
- Compare setups: Non-immobilized at 75°C avoids diffusion barriers, yielding highest product before any denaturation.
Why C is correct:
- Non-immobilized at 75°C enables unrestricted enzyme-substrate collisions at optimal temperature, maximizing velocity per Michaelis-Menten kinetics (V = V_max [S] / (K_m + [S])).
Why the others are wrong:
- A: Immobilized at 37°C faces diffusion limits and suboptimal temperature, lowering rate.
- B: Non-immobilized at 37°C operates below optimal temperature, reducing overall yield.
- D: Immobilized at 75°C benefits from stability but diffusion barriers restrict effective activity.
Final answer: C
Topic: Factors that affect enzyme action
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