Hybridoma Production via Cell Fusion

Steps:

• Recall hybridomas produce monoclonal antibodies for research and therapy.
• Identify key components: antibody-secreting B-lymphocytes fused with immortal myeloma cancer cells.
• Note B-lymphocytes generate specific antibodies post-antigen exposure.
• Match to options: only B describes the correct B-cell fusion.

Why B is correct:

• Hybridomas form by fusing antigen-activated B-lymphocytes with myeloma cells, combining antibody production with indefinite cell division (standard immunology definition).

Why the others are wrong:

• A: T-lymphocytes mediate cellular immunity, not antibody production, so fusion yields no monoclonal antibodies.
• C: Memory B-lymphocytes store antigen memory but are not the primary cells used; activated B cells (plasma precursors) are standard.
• D: T-lymphocytes lack antibody secretion, making fusion ineffective for hybridoma purpose.

Final answer: B