O Levels Biology (5090)•5090/12/O/N/23

Explanation
Optimal conditions for yeast anaerobic respiration
Steps:
- Anaerobic respiration in yeast ferments sugar to produce CO2, requiring no oxygen.
- Yeast enzymes work best at 40°C, not higher temperatures that denature proteins.
- Adding oil creates an anaerobic environment by blocking oxygen diffusion.
- Without oil, oxygen allows aerobic respiration, reducing CO2 from fermentation.
Why A is correct:
- Oil ensures anaerobic conditions while 40°C optimizes yeast fermentation of sugar to CO2, as per the equation: C6H12O6 → 2C2H5OH + 2CO2.
Why the others are wrong:
- B: 100°C denatures yeast enzymes, stopping respiration.
- C: No oil permits oxygen entry, favoring aerobic respiration over CO2-producing fermentation.
- D: No oil allows oxygen, and 100°C kills yeast, preventing any respiration.
Final answer: A
Topic: Anaerobic respiration
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