O Levels Biology (5090)•5090/12/O/N/20

Explanation
Nitrifying bacteria convert ammonium to nitrates in soil
Steps:
- Recall the nitrogen cycle: atmospheric nitrogen fixed into compounds, then transformed by soil bacteria.
- Identify nitrifying bacteria's role: they oxidize ammonium (NH4+) to nitrites (NO2-) and then nitrates (NO3-).
- Match to choices: process involves ammonium in soil becoming nitrates in soil.
- Confirm: this nitrification stage enables plant uptake of nitrates.
Why B is correct:
- Nitrifying bacteria (Nitrosomonas and Nitrobacter) perform oxidation of ammonium to nitrates, as per the biochemical process: NH4+ → NO2- → NO3-.
Why the others are wrong:
- A: Ammonium compounds are the starting substrate, not the stage carried out by nitrifying bacteria.
- C: Nitrogen in air is fixed by lightning or bacteria like Rhizobium, unrelated to nitrifiers.
- D: Nitrogen compounds in plants result from nitrate uptake and assimilation, not bacterial nitrification.
Final answer: B
Topic: Nutrient cycles
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