O Levels Biology (5090)•5090/12/O/N/18

Explanation
Yoghurt Production Sequence Steps:
- Pasteurize milk (4) to eliminate pathogens and ensure safety.
- Add bacterial culture (3) to the warm pasteurized milk for fermentation.
- Incubate at 40-45°C for 3 hours (2) to allow bacteria to convert lactose to lactic acid, thickening the milk.
- Rapidly cool the mixture (1) to stop fermentation and set the yoghurt texture.
Why D is correct:
- D follows the standard yoghurt process: pasteurization sterilizes milk before inoculation (3), incubation (2) enables bacterial activity, and cooling (1) preserves the product, per food microbiology principles.
Why the others are wrong:
- A: Begins with cooling (1), but unpasteurized milk risks contamination.
- B: Incubates (2) before pasteurization (4), allowing unwanted microbes to grow.
- C: Adds bacteria (3) before pasteurization (4), killing the culture via heat.
Final answer: D
Topic: Biotechnology
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