O Levels Biology (5090)•5090/11/M/J/24

Explanation
Amylase digests starch most effectively without pH interference
Steps:
- Amylase enzyme breaks down starch in agar into simpler sugars, creating a starch-free zone.
- Hydrochloric acid lowers pH, potentially denaturing enzymes like amylase.
- Gastric protease targets proteins, not carbohydrates like starch.
- Compare zones: largest clearance indicates most starch digestion.
Why A is correct:
- Amylase specifically catalyzes starch hydrolysis via glycosidic bond cleavage, optimal at neutral pH.
Why the others are wrong:
- B: Acid denatures amylase, inhibiting starch breakdown.
- C: Protease digests proteins, leaving starch intact.
- D: Protease ineffective on starch; acid provides no benefit for carb digestion.
Final answer: A
Topic: Effects of temperature and pH
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