O Levels Biology (5090)•5090/12/M/J/23

Explanation
Interpreting Biuret and Benedict's Test Results
Steps:
- Biuret test produces purple color with proteins due to peptide bond reaction.
- Benedict's test remains blue without reducing sugars like glucose, indicating absence.
- Purple Biuret result confirms protein presence in solution.
- Blue Benedict's result confirms no glucose in solution.
Why A is correct:
- Biuret test turns purple specifically for proteins (via copper ion coordination with peptide bonds), while Benedict's stays blue without reducing sugars like glucose (no reduction of Cu²⁺ to Cu₂O).
Why the others are wrong:
- B: Incomplete and ignores Benedict's negative result for glucose, but assumes protein only partially.
- C: Contradicts Biuret's positive protein result and misinterprets Benedict's as positive for glucose.
- D: Ignores Biuret's clear positive indication of protein.
Final answer: A
Topic: Biological molecules
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