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O Levels Biology (5090)•5090/12/M/J/23
Question 5 from 5090/12/M/J/23

Explanation

Interpreting Biuret and Benedict's Test Results

Steps:

  • Biuret test produces purple color with proteins due to peptide bond reaction.
  • Benedict's test remains blue without reducing sugars like glucose, indicating absence.
  • Purple Biuret result confirms protein presence in solution.
  • Blue Benedict's result confirms no glucose in solution.

Why A is correct:

  • Biuret test turns purple specifically for proteins (via copper ion coordination with peptide bonds), while Benedict's stays blue without reducing sugars like glucose (no reduction of Cu²⁺ to Cu₂O).

Why the others are wrong:

  • B: Incomplete and ignores Benedict's negative result for glucose, but assumes protein only partially.
  • C: Contradicts Biuret's positive protein result and misinterprets Benedict's as positive for glucose.
  • D: Ignores Biuret's clear positive indication of protein.

Final answer: A

Topic: Biological molecules

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