O Levels Biology (5090)•5090/12/M/J/23

Explanation
Nitrogen Cycle Stage Identification
Steps:
- Recall the nitrogen cycle: atmospheric N2 is converted to usable forms by bacteria, plants, animals, and back to N2.
- Locate stage X: it depicts conversion of gaseous N2 into ammonia (NH3) by symbiotic or free-living bacteria.
- Match to processes: this initial step is nitrogen fixation, not later conversions or breakdowns.
- Confirm: fixation enables plants to absorb nitrogen for growth.
Why C is correct:
- Nitrogen fixation is the biological process where prokaryotes (e.g., Rhizobium) reduce N2 to NH3 using nitrogenase enzyme, per the reaction N2 + 8H+ + 8e- → 2NH3 + H2.
Why the others are wrong:
- A: Denitrification converts nitrates back to N2 gas by bacteria in anaerobic conditions, a final stage.
- B: Decomposition breaks down organic matter, releasing ammonia, but not directly fixing atmospheric N2.
- D: Nitrification oxidizes ammonia to nitrites/nitrates by soil bacteria like Nitrosomonas, occurring after fixation.
Final answer: C
Topic: Nutrient cycles
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