O Levels Biology (5090)•5090/12/M/J/22

Explanation
Iodine Staining in Plant Cells Steps:
- Iodine solution tests for presence of starch in cells.
- Starch grains form a blue-black color with iodine due to chemical reaction.
- Cell walls consist of cellulose, which does not react with iodine.
- Cell walls retain the natural orange-brown hue of the iodine solution.
Why D is correct:
- Iodine reacts specifically with starch to produce blue-black color, while cellulose in cell walls remains orange-brown, as per the standard iodine-starch test.
Why the others are wrong:
- A: Cell walls do not stain blue-black; only starch does.
- B: Starch grains stain blue-black, not orange-brown; colors are reversed.
- C: Starch grains turn blue-black, not orange-brown.
Final answer: D
Topic: Cell structure and function
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