O Levels Biology (5090)•5090/12/M/J/21

Explanation
Non-boiled seeds show no starch reaction due to active enzyme digestion
Steps:
- Non-boiled bean seeds contain living cells with active enzymes.
- Amylase in non-boiled seeds hydrolyzes starch into sugars.
- Iodine test on non-boiled seeds yields no blue-black color as starch is absent.
- Boiled seeds denature amylase, preserving starch for positive iodine reaction.
Why D is correct:
- D explains active amylase prevents starch accumulation, per enzyme catalysis definition where non-denatured proteins function in vivo.
Why the others are wrong:
- A: Iodine absorption unrelated to lack of color change; test detects starch presence.
- B: Acid content does not affect iodine-starch reaction specificity.
- C: Amylase presence alone insufficient without noting its activity in non-boiled state.
Not enough information on exact results or full choice D text.
Final answer: D
Topic: Enzyme action
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