O Levels Biology (5090)•5090/11/M/J/21

Explanation
Non-boiled bean seeds show active enzyme function in starch digestion experiment
Steps:
- Non-boiled seeds retain living enzymes like amylase, unlike boiled ones where heat denatures proteins.
- In the presence of starch and iodine, active amylase breaks down starch into sugars.
- Iodine fails to produce a blue-black color with digested starch, explaining the lack of reaction.
- This demonstrates enzyme activity in viable seeds.
Why D is correct:
- D explains the absence of starch-iodine reaction due to enzymatic digestion, per the biological definition of amylase as a starch-hydrolyzing enzyme.
Why the others are wrong:
- A: Iodine absorption does not prevent color change; it binds to intact starch.
- B: Acid presence does not affect iodine-starch test specificity.
- C: While true, amylase alone does not fully account for the result without context of starch breakdown.
Not enough information on exact results or full choice D text to confirm.
Final answer: D
Topic: Enzyme action
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