O Levels Biology (5090)•5090/12/M/J/20

Explanation
Oil layer excludes oxygen in anaerobic fermentation
Steps:
- Fermentation by yeast produces alcohol under anaerobic conditions.
- Oxygen presence allows yeast to perform aerobic respiration instead, oxidizing alcohol.
- The oil layer floats on the mixture, forming a barrier against air.
- This ensures oxygen exclusion, promoting alcohol fermentation.
Why B is correct:
- Fermentation is defined as anaerobic respiration in yeast, yielding ethanol and CO2; oil prevents oxygen entry, blocking aerobic respiration (C6H12O6 → 2C2H5OH + 2CO2).
Why the others are wrong:
- A: Alcohol dissolves naturally in the aqueous solution; oil does not retain it.
- C: Carbon dioxide is a byproduct of yeast metabolism, not produced by oil.
- D: Oil is inert and provides no nutrients; yeast uses sugars in the mixture.
Final answer: B
Topic: Anaerobic respiration
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