O Levels Biology (5090)•5090/12/M/J/19

Explanation
Enzyme activity peaks at optimal temperature
Steps:
- Recall that enzymes like amylase have an optimal temperature where reaction rate is highest.
- Rate increases with temperature up to the optimum due to more kinetic energy and collisions.
- Beyond optimum, rate decreases as enzyme denatures and active site changes shape.
- Identify graph showing rise to peak then sharp decline as correct for amylase.
Why B is correct:
- Matches enzyme kinetics: rate rises to optimum (~37°C for human amylase) then falls due to denaturation, per Arrhenius equation adapted for proteins.
Why the others are wrong:
- A shows linear increase, ignoring denaturation.
- C shows constant rate, defying temperature effects on collisions.
- D shows steady decline, typical of non-enzymatic reactions only.
Final answer: B
Topic: Effects of temperature and pH
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